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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis using 68256-1-Ig (same clone as 68256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis using 68256-1-Ig (same clone as 68256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis of Jurkat using 68256-1-Ig (same clone as 68256-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNX17 Monoclonal antibody (68256-1-Ig)

    IF Staining of HepG2 using 68256-1-Ig (same clone as 68256-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX17 Monoclonal antibody (68256-1-Ig)

    IF Staining of HeLa using 68256-1-Ig (same clone as 68256-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

SNX17 Monoclonal antibody, PBS Only

SNX17 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68256-1-PBS
Clone No.3B8F6

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0064, SNX17, sorting nexin 17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68256-1-PBS targets SNX17 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag32624

Product name: Recombinant human SNX17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-226 aa of BC002524

Sequence: GSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYA

相同性解析による交差性が予測される生物種
Full Name sorting nexin 17
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002524
Gene Symbol SNX17
Gene ID (NCBI) 9784
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ15036
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

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