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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis using 68256-1-Ig (same clone as 68256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis using 68256-1-Ig (same clone as 68256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SNX17 Monoclonal antibody (68256-1-Ig)

    WB analysis of Jurkat using 68256-1-Ig (same clone as 68256-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNX17 Monoclonal antibody (68256-1-Ig)

    IHC staining of mouse brain using 68256-1-Ig (same clone as 68256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68256-1-Ig (SNX17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNX17 Monoclonal antibody (68256-1-Ig)

    IHC staining of mouse brain using 68256-1-Ig (same clone as 68256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68256-1-Ig (SNX17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNX17 Monoclonal antibody (68256-1-Ig)

    IF Staining of HepG2 using 68256-1-Ig (same clone as 68256-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX17 Monoclonal antibody (68256-1-Ig)

    IF Staining of HeLa using 68256-1-Ig (same clone as 68256-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68256-1-PBS in a different storage buffer formulation.

SNX17 Monoclonal antibody, PBS Only

SNX17 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68256-1-PBS
Clone No.3B8F6

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

3B8F6, KIAA0064, Sorting nexin-17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68256-1-PBS targets SNX17 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag32624

Product name: Recombinant human SNX17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-226 aa of BC002524

Sequence: GSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYA

相同性解析による交差性が予測される生物種
Full Name sorting nexin 17
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002524
Gene Symbol SNX17
Gene ID (NCBI) 9784
RRIDAB_2935341
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ15036
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

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