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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX12 Polyclonal antibody (12374-1-AP)

    WB analysis using 12374-1-AP (same clone as 12374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12374-1-AP (SNX12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12374-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX12 Polyclonal antibody (12374-1-AP)

    IF Staining of HeLa using 12374-1-AP (same clone as 12374-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (12374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12374-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12374-1-PBS targets SNX12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX12 fusion protein Ag3014 相同性解析による交差性が予測される生物種
Full Name sorting nexin 12
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC020559
Gene Symbol SNX12
Gene ID (NCBI) 29934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMY4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).