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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX12 Polyclonal antibody (12374-1-AP)

    WB analysis using 12374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12374-1-AP (SNX12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX12 Polyclonal antibody (12374-1-AP)

    IF Staining of HeLa using 12374-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (12374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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SNX12 Polyclonal antibody

SNX12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12374-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Sorting nexin-12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12374-1-AP targets SNX12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX12 fusion protein Ag3014 相同性解析による交差性が予測される生物種
Full Name sorting nexin 12
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC020559
Gene Symbol SNX12
Gene ID (NCBI) 29934
RRIDAB_3085392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

Protocols

Product Specific Protocols
WB protocol for SNX12 antibody 12374-1-APDownload protocol
IF protocol for SNX12 antibody 12374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols