Validation Data Gallery
Product Information
SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.
| Description | The SNAP/CLIP-tag-Trap Agarose Kit contains SNAP/CLIP-tag-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of SNAP-tag and CLIP-tag fusion proteins and their interacting factors.
• SNAP/CLIP-tag-Trap immunoprecipitates SNAP-tag- and Clip-tag- fusion proteinseven when already covalently bound to ligands such as dyes, biotin etc. • Bound SNAP- or CLIP-tag fusion proteins can be eluted without protease • SNAP/CLIP-tag-Trap can be used for affinity purification • Structure and function are characterized |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | SNAP-tag, CLIP-tag |
| Binding capacity | 7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 nM |
| Compatibility with mass spectrometry | The SNAP/CLIP-tag®-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2827598 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| SNAP/CLIP-tag-Trap Agarose beads | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| wtak_SDS_SNAP_CLIP-tag®-Trap Agarose Kit (EN) |
| Datasheet |
|---|
| Protocol SNAP- CLIP-tag?-Trap Agarose Kit (PDF) |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (IP) (PDF) |