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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-13 cells using SMOX Polyclonal antibody (15052-1-AP)

    WB analysis of PC-13 using 15052-1-AP (same clone as 15052-1-PBS)

    PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMOX Polyclonal antibody (15052-1-AP)

    WB analysis using 15052-1-AP (same clone as 15052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SMOX Polyclonal antibody (15052-1-AP)

    WB analysis of MCF-7 using 15052-1-AP (same clone as 15052-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human prostate cancer using 15052-1-AP (same clone as 15052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human prostate cancer using 15052-1-AP (same clone as 15052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human stomach cancer using 15052-1-AP (same clone as 15052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human stomach cancer using 15052-1-AP (same clone as 15052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human gliomas using 15052-1-AP (same clone as 15052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SMOX Polyclonal antibody (15052-1-AP)

    IHC staining of human gliomas using 15052-1-AP (same clone as 15052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15052-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SMOX Polyclonal antibody, PBS Only

SMOX Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15052-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15052-1-PBS targets SMOX in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7035

Product name: Recombinant human SMOX protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-280 aa of BC000669

Sequence: MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAAKALLEQGFTDVTVLEASSHIGGRVQSVKLGHATFELGATWIHGSHGNPIYHLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNHGRRIPKDVVEEFSDLYNEVYNLTQEFFRHDKPVNAESQNSVGVFTREEVRNRIRNDPDDPEATKRLKLAMIQQYLKVESCESSSHSMDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPAHVIQLGKPVRCIHWDQASARPRGPEIEP

相同性解析による交差性が予測される生物種
Full Name spermine oxidase
Calculated molecular weight 62 kDa
Observed molecular weight 56-65 kDa
GenBank accession numberBC000669
Gene Symbol SMOX
Gene ID (NCBI) 54498
RRIDAB_2239689
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWM0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

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