Validation Data Gallery

Filter: AllWBIHC

WB analysis of PC-13 using 15052-1-AP
PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 15052-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15052-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 15052-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 15052-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 15052-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 15052-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 15052-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 15052-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SMOX Polyclonal antibody

SMOX Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15052-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, PC-13 cells, MCF-7 cells, HeLa cells, PC-3 cells
Positive IHC detected in	human prostate cancer tissue, human stomach cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 28 publications below
IHC	See 8 publications below
IF	See 4 publications below

Product Information

15052-1-AP targets SMOX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMOX fusion protein Ag7035 相同性解析による交差性が予測される生物種
Full Name	spermine oxidase
Calculated molecular weight	62 kDa
Observed molecular weight	56-65 kDa
GenBank accession number	BC000669
Gene Symbol	SMOX
Gene ID (NCBI)	54498
RRID	AB_2239689
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NWM0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for SMOX antibody 15052-1-AP	Download protocol
IHC protocol for SMOX antibody 15052-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma. Authors - Anna Bianchi-Smiraglia View Article
mouse	WB	J Clin Invest Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology. Authors - Leslie A Sandusky-Beltran View Article
mouse	WB	EBioMedicine Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke. Authors - Guangtian Wang View Article
human	WB	J Invest Dermatol The Polyamine Putrescine Promotes Human Epidermal Melanogenesis. Authors - Aishwarya Sridharan View Article
		Free Radic Biol Med Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model. Authors - Roberta Ceci View Article
human	WB,IHC	Biomedicines Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients. Authors - Sooyoun Kim View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of PC-13 using 15052-1-AP

WB analysis using 15052-1-AP

WB analysis of MCF-7 using 15052-1-AP

IHC staining of human prostate cancer using 15052-1-AP

IHC staining of human prostate cancer using 15052-1-AP

IHC staining of human stomach cancer using 15052-1-AP

IHC staining of human stomach cancer using 15052-1-AP

IHC staining of human gliomas using 15052-1-AP

IHC staining of human gliomas using 15052-1-AP

SMOX Polyclonal antibody

SMOX Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma.

Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.

The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.

Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model.

Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients.