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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated SMO Monoclonal antib (CL488-66851-2)

    IF Staining of hTERT-RPE1 using CL488-66851-2

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 SMO antibody (CL488-66851, Clone: 2B10A11 ) at dilution of 1:200, ARL13B antibody (17711-1-AP, red).

CoraLite® Plus 488-conjugated SMO Monoclonal antibody

SMO Monoclonal Antibody for IF/ICC
Cat No. CL488-66851-2
Clone No.2A11A10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66851-2 targets SMO in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25861

Product name: Recombinant human SMO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-233 aa of BC009989

Sequence: RGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSY

相同性解析による交差性が予測される生物種
Full Name smoothened homolog (Drosophila)
Calculated molecular weight 787 aa, 86 kDa
GenBank accession numberBC009989
Gene Symbol SMO
Gene ID (NCBI) 6608
RRIDAB_3084278
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ99835
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SMO antibody CL488-66851-2Download protocol
Standard Protocols
Click here to view our Standard Protocols