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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMC4 Recombinant monoclonal antibody (86182-1-RR)

    WB analysis using 86182-1-RR (same clone as 86182-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86182-1-RR (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86182-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMC4 Recombinant monoclonal antibody (86182-1-RR)

    IF Staining of HeLa using 86182-1-RR (same clone as 86182-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMC4 antibody (86182-1-RR, Clone: 250782E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86182-1-PBS in a different storage buffer formulation.

SMC4 Recombinant monoclonal antibody, PBS Only

SMC4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86182-1-PBS
Clone No.250782E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86182-1-PBS targets SMC4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19156

Product name: Recombinant human SMC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 992-1288 aa of BC106033

Sequence: SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNII

相同性解析による交差性が予測される生物種
Full Name structural maintenance of chromosomes 4
Calculated molecular weight 1288 aa, 147 kDa
Observed molecular weight170-180 kDa
GenBank accession numberBC106033
Gene Symbol SMC4
Gene ID (NCBI) 10051
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NTJ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.

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