Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86015-1-RR targets SMARCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SMARCC1 fusion protein Ag12109 相同性解析による交差性が予測される生物種 |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 |
| Calculated molecular weight | 1105 aa, 123 kDa |
| Observed molecular weight | 155-160 kDa |
| GenBank accession number | BC050564 |
| Gene Symbol | SMARCC1 |
| Gene ID (NCBI) | 6599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92922 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCC1 antibody 86015-1-RR | Download protocol |
| IF protocol for SMARCC1 antibody 86015-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |