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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMARCC1 Recombinant antibody (86015-1-RR)

    WB analysis using 86015-1-RR (same clone as 86015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86015-1-RR (SMARCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

  • Sample test of MP02222-1

    Sample test of MP02222-1

    The mean SMARCC1 concentration was determined to be 130.2 ng/mL in Jurkat cell extract based on a 2.2 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using SMARCC1 Recombinant antibody (86015-1-RR)

    IF Staining of Jurkat using 86015-1-RR (same clone as 86015-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMARCC1 antibody (86015-1-RR, Clone: 250455A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02222-1

    Cytometric bead array standard curve of MP02222-1

    Cytometric bead array standard curve of MP02222-1, SMARCC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86015-1-PBS. Detection antibody: 86015-2-PBS. Standard: Ag12109. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 86015-1-RR

    Affinity and Kinetic Characterization of 86015-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86015-1-RR against Human SMARCC1 were performed. The affinity constant is 0.393 nM.

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SMARCC1 Recombinant antibody, PBS Only (Capture)

SMARCC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 86015-1-PBS
Clone No.250455A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86015-1-PBS targets SMARCC1 as part of a matched antibody pair:

MP02222-1: 86015-1-PBS capture and 86015-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMARCC1 fusion protein Ag12109 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
Calculated molecular weight 1105 aa, 123 kDa
Observed molecular weight155-160 kDa
GenBank accession numberBC050564
Gene Symbol SMARCC1
Gene ID (NCBI) 6599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92922
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)