CoraLite® Plus 488-conjugated SMARCB1 Monoclonal antibody

SMARCB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D10A4

Cat no : CL488-67979

Synonyms

BAF47, BRG1 associated factor 47, hSNF5, hSNFS, INI1, Integrase interactor 1 protein, RDT, Sfh1p, SMARCB1, SNF5, SNF5 homolog, SNF5L1, Snr1



Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67979-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67979 targets SMARCB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCB1 fusion protein Ag28911 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
Calculated molecular weight 44 kDa
Observed molecular weight40-45 kDa
GenBank accession numberNM_003073
Gene symbol SMARCB1
Gene ID (NCBI) 6598
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCB1 is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SMARCB1 antibody CL488-67979Download protocol
Standard Protocols
Click here to view our Standard Protocols