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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SLC4A4 Polyclonal antibody (11885-1-AP)

    WB analysis of HEK-293 using 11885-1-AP (same clone as 11885-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC4A4 Polyclonal antibody (11885-1-AP)

    WB analysis of mouse kidney using 11885-1-AP (same clone as 11885-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11885-1-AP (SLC4A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SLC4A4 Polyclonal antibody (11885-1-AP)

    IP experiment of HEK-293 using 11885-1-AP (same clone as 11885-1-PBS)

    IP result of anti-SLC4A4 (IP:11885-1-AP, 4ug; Detection:11885-1-AP 1:400) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC4A4 Polyclonal antibody (11885-1-AP)

    IHC staining of human kidney using 11885-1-AP (same clone as 11885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SLC4A4 Polyclonal antibody (11885-1-AP)

    IHC staining of human kidney using 11885-1-AP (same clone as 11885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SLC4A4 Polyclonal antibody (11885-1-AP)

    IHC staining of mouse small intestine using 11885-1-AP (same clone as 11885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SLC4A4 Polyclonal antibody (11885-1-AP)

    IHC staining of mouse small intestine using 11885-1-AP (same clone as 11885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC4A4 Polyclonal antibody (11885-1-AP)

    IF Staining of HepG2 using 11885-1-AP (same clone as 11885-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using SLC4A4 antibody 11885-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11885-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SLC4A4 Polyclonal antibody, PBS Only

SLC4A4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11885-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11885-1-PBS targets SLC4A4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2490

Product name: Recombinant human SLC4A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-354 aa of BC030977

Sequence: LRVVQPMFNHSIFTSAVSPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLP

相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, sodium bicarbonate cotransporter, member 4
Calculated molecular weight 121 kDa
Observed molecular weight 121 kDa
GenBank accession numberBC030977
Gene Symbol SLC4A4
Gene ID (NCBI) 8671
RRIDAB_2191458
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6R1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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