- Featured Product
- KD/KO Validated
SLC4A4 Polyclonal antibody
SLC4A4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11885-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SLC4A4 Ready-To-Use IHC Kit. SLC4A4 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| IHC | See 7 publications below |
| IF | See 1 publications below |
Product Information
11885-1-AP targets SLC4A4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SLC4A4 fusion protein Ag2490 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 4, sodium bicarbonate cotransporter, member 4 |
| Calculated molecular weight | 121 kDa |
| Observed molecular weight | 121 kDa |
| GenBank accession number | BC030977 |
| Gene symbol | SLC4A4 |
| Gene ID (NCBI) | 8671 |
| RRID | AB_2191458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC4A4 antibody 11885-1-AP | Download protocol |
| IHC protocol for SLC4A4 antibody 11885-1-AP | Download protocol |
| IF protocol for SLC4A4 antibody 11885-1-AP | Download protocol |
| IP protocol for SLC4A4 antibody 11885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis. | ||
Int J Mol Sci Modulation of Tubular pH by Acetazolamide in a Ca2+ Transport Deficient Mice Facilitates Calcium Nephrolithiasis. | ||
Am J Physiol Gastrointest Liver Physiol Expression of the regulated isoform of the electrogenic Na+/HCO3- cotransporter, NBCe1, is enriched in pacemaker interstitial cells of Cajal.
| ||
Am J Physiol Renal Physiol Expression of sodium-dependent dicarboxylate transporter 1 (NaDC1/SLC13A2) in normal and neoplastic human kidney. | ||
Front Physiol Selective Deletion of the Mechanistic Target of Rapamycin From the Renal Collecting Duct Principal Cell in Mice Down-Regulates the Epithelial Sodium Channel. | ||
Front Physiol Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis. |
