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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC38A1 Polyclonal antibody (28632-1-AP)

    WB analysis using 28632-1-AP (same clone as 28632-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28632-1-AP (SLC38A1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SLC38A1 Polyclonal antibody (28632-1-AP)

    WB analysis of HeLa using 28632-1-AP (same clone as 28632-1-PBS)

    WB result of SLC38A1 antibody (28632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A1 transfected Jurkat cells. This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC38A1 Polyclonal antibody (28632-1-AP)

    IHC staining of mouse brain using 28632-1-AP (same clone as 28632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SLC38A1 Polyclonal antibody (28632-1-AP)

    IHC staining of mouse brain using 28632-1-AP (same clone as 28632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28632-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28632-1-PBS targets SLC38A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC38A1 fusion protein Ag30088 相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 1
Calculated molecular weight 487 aa, 54 kDa
Observed molecular weight54 kDa, 60-70 kDa
GenBank accession numberBC010620
Gene Symbol SLC38A1
Gene ID (NCBI) 81539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2H9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.