Validation Data Gallery

Filter: AllWBIHCFC (Intra)

WB analysis using 28632-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28632-1-AP (SLC38A1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 28632-1-AP
WB result of SLC38A1 antibody (28632-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC38A1 transfected Jurkat cells.
IHC staining of mouse brain using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 28632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28632-1-AP (SLC38A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of A549 using 28632-1-AP
1x10^6 A549 cells were intracellularly stained with 0.25 ug SLC38A1 Polyclonal antibody (28632-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Featured Product
KD/KO Validated

SLC38A1 Polyclonal antibody

SLC38A1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28632-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Amino acid transporter A1, ATA1, NAT2, N-system amino acid transporter 2, SAT1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected in	mouse brain tissue, human placenta tissue, mouse lung tissue, human ovary cancer tissue, mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

28632-1-AP targets SLC38A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag30088 Product name: Recombinant human SLC38A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-74 aa of BC010620 Sequence: MMHFKSGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSKFISDRESRRSLTNSHLEKKKCDEYIPGTTSLG 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 38, member 1
Calculated molecular weight	487 aa, 54 kDa
Observed molecular weight	54 kDa, 60-70 kDa
GenBank accession number	BC010620
Gene Symbol	SLC38A1
Gene ID (NCBI)	81539
RRID	AB_3086073
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H2H9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Protocols

Product Specific Protocols
FC protocol for SLC38A1 antibody 28632-1-AP	Download protocol
IHC protocol for SLC38A1 antibody 28632-1-AP	Download protocol
WB protocol for SLC38A1 antibody 28632-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB	Metabolism ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake. Authors - Dehui Hou View Article
mouse,human	WB,IHC,IF	Cell Rep Med Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models. Authors - Yinan Wang View Article
mouse	WB	Sci Adv Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition Authors - Qiang Yu View Article
human	WB,IHC	Cancer Lett Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer Authors - Minghua Bai View Article
human	WB	Cancer Lett HHLA2 promotes immune evasion in EGFR-mutant lung cancer by inhibiting CD8(+) T cell glutamine metabolism via KIR3DL3 interaction. Authors - Fei Wu View Article
human	WB	Biomolecules Targeting Sodium Transport Reveals CHP1 Downregulation as a Novel Molecular Feature of Malignant Progression in Clear Cell Renal Cell Carcinoma: Insights from Integrated Multi-Omics Analyses Authors - Yun Wu View Article

Validation Data Gallery

WB analysis using 28632-1-AP

WB analysis of HeLa using 28632-1-AP

IHC staining of mouse brain using 28632-1-AP

IHC staining of mouse brain using 28632-1-AP

IHC staining of mouse cerebellum using 28632-1-AP

IHC staining of human ovary cancer using 28632-1-AP

IHC staining of human placenta using 28632-1-AP

IHC staining of mouse lung using 28632-1-AP

FC experiment of A549 using 28632-1-AP

SLC38A1 Polyclonal antibody

SLC38A1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake.

Nanoparticle-based serine replenishment rescues SP1-BNIP3 mitophagy and ameliorates preeclampsia in preclinical models.

Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition

Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer

HHLA2 promotes immune evasion in EGFR-mutant lung cancer by inhibiting CD8(+) T cell glutamine metabolism via KIR3DL3 interaction.

Targeting Sodium Transport Reveals CHP1 Downregulation as a Novel Molecular Feature of Malignant Progression in Clear Cell Renal Cell Carcinoma: Insights from Integrated Multi-Omics Analyses

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