Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
Product Information
28632-1-AP targets SLC38A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30088 Product name: Recombinant human SLC38A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-74 aa of BC010620 Sequence: MMHFKSGLELTELQNMTVPEDDNISNDSNDFTEVENGQINSKFISDRESRRSLTNSHLEKKKCDEYIPGTTSLG 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 38, member 1 |
| Calculated molecular weight | 487 aa, 54 kDa |
| Observed molecular weight | 54 kDa, 60-70 kDa |
| GenBank accession number | BC010620 |
| Gene Symbol | SLC38A1 |
| Gene ID (NCBI) | 81539 |
| RRID | AB_3086073 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2H9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC38A1 antibody 28632-1-AP | Download protocol |
| WB protocol for SLC38A1 antibody 28632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal CENPA promotes glutamine metabolism and tumor progression by up-regulating SLC38A1 in endometrial cancer | ||
Cancers (Basel) GluOC Induced SLC7A11 and SLC38A1 to Activate Redox Processes and Resist Ferroptosis in TNBC
| ||
Metabolism ALDH2 deficiency aggravates vascular injury-induced restenosis by enhancing vascular smooth muscle cell proliferation through SLC38A2-mediated upregulation of glutamine uptake. | ||
Nan Fang Yi Ke Da Xue Xue Bao [Prenatal fear stress impairs cognitive development in offspring rats by disrupting placental amino acid transport]. | ||
Exp Gerontol SLC38A1 protects against aging-related oxidative stress and lipid peroxidation in C2C12 myoblasts: Implication of a ferroptosis-related regulator for skeletal muscle aging.
| ||
Discov Oncol Unveiling membrane transporter proteins-related genes for predicting the prognosis of hepatocellular carcinoma patients. |