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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated SLC35F4 Recombinant (CL488-83085-3)

    IF Staining of U2OS using CL488-83085-3

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SLC35F4 antibody (CL488-83085-3, Clone: 230360B7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated SLC35F4 Recombinant (CL488-83085-3)

    FC experiment of U2OS using CL488-83085-3

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated SLC35F4 Recombinant antibody (CL488-83085-3, Clone:230360B7)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated SLC35F4 Recombinant antibody

SLC35F4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83085-3
Clone No.230360B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

C14orf36, 230360B7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83085-3 targets SLC35F4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC35F4 fusion protein Ag34518 相同性解析による交差性が予測される生物種
Full Name solute carrier family 35, member F4
Calculated molecular weight521 aa, 58 kDa
GenBank accession numberNM_001206920
Gene Symbol SLC35F4
Gene ID (NCBI) 341880
RRIDAB_3673183
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SLC35F4 antibody CL488-83085-3Download protocol
FC protocol for CL Plus 488 SLC35F4 antibody CL488-83085-3Download protocol
Standard Protocols
Click here to view our Standard Protocols