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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC26A8 Polyclonal antibody (12776-1-AP)

    WB analysis using 12776-1-AP (same clone as 12776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12776-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SLC26A8 Polyclonal antibody (12776-1-AP)

    WB analysis of mouse testis using 12776-1-AP (same clone as 12776-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SLC26A8 Polyclonal antibody (12776-1-AP)

    IF Staining of mouse testis using 12776-1-AP (same clone as 12776-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SLC26A8 Polyclonal antibody (12776-1-AP)

    IF Staining of mouse testis using 12776-1-AP (same clone as 12776-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12776-1-PBS in a different storage buffer formulation.

SLC26A8 Polyclonal antibody, PBS Only

SLC26A8 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 12776-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12776-1-PBS targets SLC26A8 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3472

Product name: Recombinant human SLC26A8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 570-864 aa of BC025408

Sequence: QQYEEVEEVWLPNNSSRNSSPGLPDVAESQGRRSLIPYSDASLLPSVHTIILDFSMVHYVDSRGLVVLRQICNAFQNANILILIAGCHSSIVRAFERNDFFDAGITKTQLFLSVHDAVLFALSRKVIGSSELSIDESETVIRETYSETDKNDNSRYKMSSSFLGSQKNVSPGFIKIQQPVEEESELDLELESEQEAGLGLDLDLDRELEPEMEPKAETETKTQTEMEPQPETEPEMEPNPKSRPRAHTFPQQRYWPMYHPSMASTQSQTQTRTWSVERRRHPMDSYSPEGNSNED

相同性解析による交差性が予測される生物種
Full Name solute carrier family 26, member 8
Calculated molecular weight 970 aa, 109 kDa
Observed molecular weight 62, 86 and 97 kDa
GenBank accession numberBC025408
Gene Symbol SLC26A8
Gene ID (NCBI) 116369
RRIDAB_2877880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RN1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SLC26A8, also named as TAT1, acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. It has been reported that SLC26A8 is a sulfate transporter specifically expressed in spermatocytes and spermatids, playing a key role in spermatogenesis. There are several isoforms of SLC26A8 and 12776-1-AP antibody detects 62, 86 and 97 kDa protein in SDS-PAGE. (PMID: 11278976; 11834742)

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