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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC26A8 Polyclonal antibody (12776-1-AP)

    WB analysis using 12776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SLC26A8 Polyclonal antibody (12776-1-AP)

    WB analysis of mouse testis using 12776-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SLC26A8 Polyclonal antibody (12776-1-AP)

    IF Staining of mouse testis using 12776-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SLC26A8 Polyclonal antibody (12776-1-AP)

    IF Staining of mouse testis using 12776-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SLC26A8 Polyclonal antibody

SLC26A8 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 12776-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

TAT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12776-1-AP targets SLC26A8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC26A8 fusion protein Ag3472 相同性解析による交差性が予測される生物種
Full Name solute carrier family 26, member 8
Calculated molecular weight 970 aa, 109 kDa
Observed molecular weight 62, 86 and 97 kDa
GenBank accession numberBC025408
Gene Symbol SLC26A8
Gene ID (NCBI) 116369
RRIDAB_2877880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC26A8, also named as TAT1, acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. It has been reported that SLC26A8 is a sulfate transporter specifically expressed in spermatocytes and spermatids, playing a key role in spermatogenesis. There are several isoforms of SLC26A8 and 12776-1-AP antibody detects 62, 86 and 97 kDa protein in SDS-PAGE. (PMID: 11278976; 11834742)

Protocols

Product Specific Protocols
WB protocol for SLC26A8 antibody 12776-1-APDownload protocol
IF protocol for SLC26A8 antibody 12776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

The role of intestinal immune cells and matrix metalloproteinases in inflammatory bowel disease

Authors - Kun Mei
View Article
humanIF

Asian J Androl

The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities

Authors - Bin Mao
View Article