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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using SIX6 Monoclonal antibody (67622-1-Ig)

    WB analysis of HUVEC using 67622-1-Ig (same clone as 67622-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67622-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using SIX6 Monoclonal antibody (67622-1-Ig)

    WB analysis of NCCIT using 67622-1-Ig (same clone as 67622-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67622-1-PBS in a different storage buffer formulation.

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SIX6 Monoclonal antibody, PBS Only

SIX6 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67622-1-PBS
Clone No.1C12E2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

Homeobox protein SIX6, Homeodomain protein OPTX2, MCOPCT2, Optic homeobox 2, OPTX2, Sine oculis homeobox homolog 6, SIX homeobox 6, SIX6, Six9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67622-1-PBS targets SIX6 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIX6 fusion protein Ag15496 相同性解析による交差性が予測される生物種
Full Name SIX homeobox 6
Calculated molecular weight 246 aa, 28 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC069413
Gene Symbol SIX6
Gene ID (NCBI) 4990
RRIDAB_2882824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95475
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SIX6, also named as OPTX2 or SIX9, is a 246 amino acid protein, which is expressed in the developing and adult retina. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).