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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using SIX6 Monoclonal antibody (67622-1-Ig)

    WB analysis of HUVEC using 67622-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using SIX6 Monoclonal antibody (67622-1-Ig)

    WB analysis of NCCIT using 67622-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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SIX6 Monoclonal antibody

SIX6 Monoclonal Antibody for WB, ELISA
Cat No. 67622-1-Ig
Clone No.1C12E2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

1C12E2, Homeobox protein SIX6, OPTX2, Six9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells, NCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67622-1-Ig targets SIX6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIX6 fusion protein Ag15496 相同性解析による交差性が予測される生物種
Full Name SIX homeobox 6
Calculated molecular weight 246 aa, 28 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC069413
Gene Symbol SIX6
Gene ID (NCBI) 4990
RRIDAB_2882824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95475
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIX6, also named as OPTX2 or SIX9, is a 246 amino acid protein, which is expressed in the developing and adult retina. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).

Protocols

Product Specific Protocols
WB protocol for SIX6 antibody 67622-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols