Validation Data Gallery
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Tested Applications
| Positive WB detected in | HUVEC cells, NCCIT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67622-1-Ig targets SIX6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SIX6 fusion protein Ag15496 相同性解析による交差性が予測される生物種 |
| Full Name | SIX homeobox 6 |
| Calculated molecular weight | 246 aa, 28 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC069413 |
| Gene Symbol | SIX6 |
| Gene ID (NCBI) | 4990 |
| RRID | AB_2882824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIX6, also named as OPTX2 or SIX9, is a 246 amino acid protein, which is expressed in the developing and adult retina. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIX6 antibody 67622-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia |