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Tested Applications
| Positive FC detected in | human peripheral blood leukocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-98267 targets SIRP beta 1/CD172b in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SIRP beta 1/CD172b fusion protein Eg1668 相同性解析による交差性が予測される生物種 |
| Full Name | signal-regulatory protein beta 1 |
| Calculated molecular weight | 43kDa |
| GenBank accession number | NM_006065.5 |
| Gene Symbol | SIRPB1 |
| Gene ID (NCBI) | 10326 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00241-1 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
SIRP beta 1, also known as CD172b, is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP beta 1 also participates in the recruitment of tyrosine kinase SYK.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 SIRP beta 1/CD172b antibody CL647-98267 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |