Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells, MCF-7 cells, Jurkat cells, A431 cells, rat liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85758-5-RR targets SHMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag31269 Product name: Recombinant human SHMT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 320-483 aa of BC038598 Sequence: MTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF 相同性解析による交差性が予測される生物種 |
| Full Name | serine hydroxymethyltransferase 1 (soluble) |
| Calculated molecular weight | 483 aa, 53 kDa |
| Observed molecular weight | 45-53 kDa |
| GenBank accession number | BC038598 |
| Gene Symbol | SHMT1 |
| Gene ID (NCBI) | 6470 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P34896 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SHMT1 antibody 85758-5-RR | Download protocol |
| WB protocol for SHMT1 antibody 85758-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
