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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHMT1 Recombinant antibody (85758-5-RR)

    WB analysis using 85758-5-RR (same clone as 85758-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85758-5-RR (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SHMT1 Recombinant antibody (85758-5-RR)

    WB analysis using 85758-5-RR (same clone as 85758-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85758-5-RR (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHMT1 Recombinant antibody (85758-5-RR)

    IF Staining of HepG2 using 85758-5-RR (same clone as 85758-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT1 antibody (85758-5-RR, Clone: 243008E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.

SHMT1 Recombinant monoclonal antibody, PBS Only

SHMT1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85758-5-PBS
Clone No.243008E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CSHMT, EC:2.1.2.1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85758-5-PBS targets SHMT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag31269

Product name: Recombinant human SHMT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 320-483 aa of BC038598

Sequence: MTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF

相同性解析による交差性が予測される生物種
Full Name serine hydroxymethyltransferase 1 (soluble)
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight45-53 kDa
GenBank accession numberBC038598
Gene Symbol SHMT1
Gene ID (NCBI) 6470
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP34896
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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