• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SHC1 Monoclonal antibody (67194-2-Ig)

    WB analysis using 67194-2-Ig (same clone as 67194-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67194-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of hTERT-RPE1 cells using SHC1 Monoclonal antibody (67194-2-Ig)

    WB analysis of hTERT-RPE1 using 67194-2-Ig (same clone as 67194-2-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67194-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50763-1

    Cytometric bead array standard curve of MP50763-1

    Cytometric bead array standard curve of MP50763-1, SHC1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67194-2-PBS. Detection antibody: 67194-3-PBS. Standard:Ag25173. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50763-3

    Cytometric bead array standard curve of MP50763-3

    Cytometric bead array standard curve of MP50763-3, SHC1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67194-2-PBS. Detection antibody: 67194-4-PBS. Standard:Ag25173. Range: 0.391-50 ng/mL.

SHC1 Monoclonal antibody, PBS Only (Capture)

SHC1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67194-2-PBS
Clone No.1A5B1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

SHC, 1A5B1, SH2 domain protein C1, SHC transforming protein 1, SHC-transforming protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67194-2-PBS targets SHC1 as part of a matched antibody pair:

MP50763-1: 67194-2-PBS capture and 67194-3-PBS detection (validated in Cytometric bead array)

MP50763-3: 67194-2-PBS capture and 67194-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25173

Product name: Recombinant human SHC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-169 aa of NM_183001

Sequence: MDLLPPKPKYNPLRNESLSSLEEGASGSTPPEELPSPSASSLGPILPPLPGDDSPTTLCSFFPRMSNLRLANPAGGRPGSKGEPGRAADDGEGIVGAAMPDSGPLPLLQDMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGWLHPNDKVMGPGVSYLVRYMGC

相同性解析による交差性が予測される生物種
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Calculated molecular weight 63 kDa
Observed molecular weight66 kDa
GenBank accession numberNM_183001
Gene Symbol SHC1
Gene ID (NCBI) 6464
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29353
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.