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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHC1 Monoclonal antibody (67194-2-Ig)

    WB analysis using 67194-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of hTERT-RPE1 cells using SHC1 Monoclonal antibody (67194-2-Ig)

    WB analysis of hTERT-RPE1 using 67194-2-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

SHC1 Monoclonal antibody

SHC1 Monoclonal Antibody for WB, ELISA
Cat No. 67194-2-Ig
Clone No.1A5B1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

SHC, 1A5B1, SH2 domain protein C1, SHC transforming protein 1, SHC-transforming protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTT cells, hTERT-RPE1 cells, PANC-1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67194-2-Ig targets SHC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25173

Product name: Recombinant human SHC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-169 aa of NM_183001

Sequence: MDLLPPKPKYNPLRNESLSSLEEGASGSTPPEELPSPSASSLGPILPPLPGDDSPTTLCSFFPRMSNLRLANPAGGRPGSKGEPGRAADDGEGIVGAAMPDSGPLPLLQDMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGWLHPNDKVMGPGVSYLVRYMGC

相同性解析による交差性が予測される生物種
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Calculated molecular weight 63 kDa
Observed molecular weight66 kDa
GenBank accession numberNM_183001
Gene Symbol SHC1
Gene ID (NCBI) 6464
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29353
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Protocols

Product Specific Protocols
WB protocol for SHC1 antibody 67194-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols