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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHC1 Polyclonal antibody (12496-1-AP)

    WB analysis using 12496-1-AP (same clone as 12496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SHC1 Polyclonal antibody (12496-1-AP)

    WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SHC1 Polyclonal antibody (12496-1-AP)

    WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SHC1 Polyclonal antibody (12496-1-AP)

    IHC staining of human stomach using 12496-1-AP (same clone as 12496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SHC1 Polyclonal antibody (12496-1-AP)

    IF Staining of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHC1 Polyclonal antibody (12496-1-AP)

    IF Staining of HepG2 using 12496-1-AP (same clone as 12496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SHC1 Polyclonal antibody, PBS Only

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12496-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12496-1-PBS targets SHC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3165

Product name: Recombinant human SHC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 175-474 aa of BC014158

Sequence: RACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQAVGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWFHGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL

相同性解析による交差性が予測される生物種
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa, 52 kDa, 46 kDa
GenBank accession numberBC014158
Gene Symbol SHC1
Gene ID (NCBI) 6464
RRIDAB_2187889
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29353
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

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