Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
12496-1-AP targets SHC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3165 Product name: Recombinant human SHC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 175-474 aa of BC014158 Sequence: RACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQAVGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWFHGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL 相同性解析による交差性が予測される生物種 |
| Full Name | SHC (Src homology 2 domain containing) transforming protein 1 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66 kDa, 52 kDa, 46 kDa |
| GenBank accession number | BC014158 |
| Gene Symbol | SHC1 |
| Gene ID (NCBI) | 6464 |
| RRID | AB_2187889 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SHC1 antibody 12496-1-AP | Download protocol |
| IHC protocol for SHC1 antibody 12496-1-AP | Download protocol |
| WB protocol for SHC1 antibody 12496-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Cell Res LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway. | ||
Sci Adv HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis | ||
Biomed Pharmacother Buparlisib and ponatinib inhibit aggressiveness of cholangiocarcinoma cells via suppression of IRS1-related pathway by targeting oxidative stress resistance |
