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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SH2D4A Polyclonal antibody (15957-1-AP)

    WB analysis of HeLa using 15957-1-AP (same clone as 15957-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15957-1-AP (SH2D4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15957-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SH2D4A Polyclonal antibody (15957-1-AP)

    IHC staining of human stomach cancer using 15957-1-AP (same clone as 15957-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15957-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SH2D4A Polyclonal antibody (15957-1-AP)

    IHC staining of human stomach cancer using 15957-1-AP (same clone as 15957-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15957-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH2D4A Polyclonal antibody (15957-1-AP)

    IF Staining of HeLa using 15957-1-AP (same clone as 15957-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH2D4A antibody (15957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15957-1-PBS in a different storage buffer formulation.

SH2D4A Polyclonal antibody, PBS Only

SH2D4A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15957-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15957-1-PBS targets SH2D4A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8713

Product name: Recombinant human SH2D4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC014525

Sequence: HSEEFTNSLKTKSQYHDLQAPDNQQTKDIWKKVAEKEELEQGSRPAPTLEEEKIRSLSSSSRNIQQMLADSINRMKAYAFHQKKESMKKKQDEEINQIEEERTKQICKSWKEDSEWQASLRKSKAADEKRRSLAKQAREDYKRLSLGAQKGRGGERLQNPLRVPQKPERPPLPPKPQFLNSGAYPQKPLRNQGVVRTLSSSAQEDIIRWFKEEQLPLRAGYQKTSDTIAPWFHGILTLKKANELLLSTGMPGSFLIRVSERIKGYALSYLSEDGCKHFLIDASADAYSFLGVDQLQHATLADLVEYHKEEPITSLGKELLLYPCGQQDQLPDYLELFE

相同性解析による交差性が予測される生物種
Full Name SH2 domain containing 4A
Calculated molecular weight 454 aa, 53 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC014525
Gene Symbol SH2D4A
Gene ID (NCBI) 63898
RRIDAB_2286025
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H788
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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