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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SH2D4A Polyclonal antibody (15957-1-AP)

    WB analysis of HeLa using 15957-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15957-1-AP (SH2D4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SH2D4A Polyclonal antibody (15957-1-AP)

    IHC staining of human stomach cancer using 15957-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SH2D4A Polyclonal antibody (15957-1-AP)

    IHC staining of human stomach cancer using 15957-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15957-1-AP (SH2D4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH2D4A Polyclonal antibody (15957-1-AP)

    IF Staining of HeLa using 15957-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH2D4A antibody (15957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SH2D4A Polyclonal antibody

SH2D4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15957-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SH2A, SH2 domain-containing protein 4A, Protein phosphatase 1 regulatory subunit 38, PPP1R38

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15957-1-AP targets SH2D4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2D4A fusion protein Ag8713 相同性解析による交差性が予測される生物種
Full Name SH2 domain containing 4A
Calculated molecular weight 454 aa, 53 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC014525
Gene Symbol SH2D4A
Gene ID (NCBI) 63898
RRIDAB_2286025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SH2D4A antibody 15957-1-APDownload protocol
IHC protocol for SH2D4A antibody 15957-1-APDownload protocol
IF protocol for SH2D4A antibody 15957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Integrated clustering signature of genomic heterogeneity, stemness and tumor microenvironment predicts glioma prognosis and immunotherapy response

Authors - Yangyang Wu
  • KD Validated
View Article
humanIHC,WB

Cell Signal

SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway

Authors - Haoming Shi
View Article