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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SH2D2A Polyclonal antibody (18539-1-AP)

    WB analysis of K-562 using 18539-1-AP (same clone as 18539-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SH2D2A Polyclonal antibody (18539-1-AP)

    IHC staining of human heart using 18539-1-AP (same clone as 18539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SH2D2A Polyclonal antibody (18539-1-AP)

    IHC staining of human heart using 18539-1-AP (same clone as 18539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using SH2D2A Polyclonal antibody (18539-1-AP)

    FC experiment of K-562 using 18539-1-AP (same clone as 18539-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug SH2D2A Polyclonal antibody (18539-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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SH2D2A Polyclonal antibody, PBS Only

SH2D2A Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 18539-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18539-1-PBS targets SH2D2A in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2D2A fusion protein Ag13355 相同性解析による交差性が予測される生物種
Full Name SH2 domain protein 2A
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC012107
Gene Symbol SH2D2A
Gene ID (NCBI) 9047
RRIDAB_10640158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP31
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.