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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH2D2A Polyclonal antibody

SH2D2A Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18539-1-AP

Synonyms

F2771, SCAP, SH2 domain protein 2A, SH2D2A, TSAd, VRAP

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 18539-1-AP

    WB analysis of K-562 using 18539-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 18539-1-AP

    IHC staining of human heart using 18539-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 18539-1-AP

    IHC staining of human heart using 18539-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of K-562 using 18539-1-AP

    FC experiment of K-562 using 18539-1-AP

    1X10^6 K-562 cells were stained with 0.2ug SH2D2A antibody (18539-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 18539-1-AP is SH2D2A Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2D2A fusion protein Ag13355 相同性解析による交差性が予測される生物種
Full Name SH2 domain protein 2A
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC012107
Gene symbol SH2D2A
Gene ID (NCBI) 9047
RRIDAB_10640158
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SH2D2A antibody 18539-1-APDownload protocol
IHC protocol for SH2D2A antibody 18539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mult Scler Relat Disord

Downregulation of lncRNA XIST may promote Th17 differentiation through KDM6A-TSAd pathway in neuromyelitis optica spectrum disorders

Authors - Ruo-Yi Guo
View Article