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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SFRS8 Polyclonal antibody (24705-1-AP)

    WB analysis of Jurkat using 24705-1-AP (same clone as 24705-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24705-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SFRS8 Polyclonal antibody (24705-1-AP)

    WB analysis of HeLa using 24705-1-AP (same clone as 24705-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24705-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SFRS8 Polyclonal antibody (24705-1-AP)

    IF Staining of HeLa using 24705-1-AP (same clone as 24705-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24705-1-AP (SFRS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24705-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24705-1-PBS targets SFRS8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS8 fusion protein Ag19762 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 8 (suppressor-of-white-apricot homolog, Drosophila)
Calculated molecular weight 951 aa, 105 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC136678
Gene Symbol SFRS8
Gene ID (NCBI) 6433
RRIDAB_2879682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12872
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SFRS8, also named as SFSWAP and SWAP, is splicing regulator. It regulates the splicing of fibronectin and CD45 genes. SFRS8 represses the splicing of MAPT/Tau exon 10. This antibody detects 130-150 kDa SFRS8 protein.