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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SFRS8 Polyclonal antibody (24705-1-AP)

    WB analysis of Jurkat using 24705-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SFRS8 Polyclonal antibody (24705-1-AP)

    WB analysis of HeLa using 24705-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SFRS8 Polyclonal antibody (24705-1-AP)

    IF Staining of HeLa using 24705-1-AP

    Immunofluorescent analysis of HeLa cells using 24705-1-AP (SFRS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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SFRS8 Polyclonal antibody

SFRS8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24705-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SFRS8, SWAP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24705-1-AP targets SFRS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS8 fusion protein Ag19762 相同性解析による交差性が予測される生物種
Full Name splicing factor, arginine/serine-rich 8 (suppressor-of-white-apricot homolog, Drosophila)
Calculated molecular weight 951 aa, 105 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC136678
Gene Symbol SFRS8
Gene ID (NCBI) 6433
RRIDAB_2879682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12872
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFRS8, also named as SFSWAP and SWAP, is splicing regulator. It regulates the splicing of fibronectin and CD45 genes. SFRS8 represses the splicing of MAPT/Tau exon 10. This antibody detects 130-150 kDa SFRS8 protein.

Protocols

Product Specific Protocols
WB protocol for SFRS8 antibody 24705-1-APDownload protocol
IF protocol for SFRS8 antibody 24705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Clin Transl Med

Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication.

Authors - Yuanjiao Zhang
View Article