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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SFI1 Polyclonal antibody (13550-1-AP)

    WB analysis of A549 using 13550-1-AP (same clone as 13550-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SFI1 Polyclonal antibody (13550-1-AP)

    WB analysis using 13550-1-AP (same clone as 13550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SFI1 Polyclonal antibody (13550-1-AP)

    WB analysis of HepG2 using 13550-1-AP (same clone as 13550-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using SFI1 Polyclonal antibody (13550-1-AP)

    WB analysis of mouse lung using 13550-1-AP (same clone as 13550-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SFI1 Polyclonal antibody (13550-1-AP)

    IF Staining of HepG2 using 13550-1-AP (same clone as 13550-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SFI1 Polyclonal antibody (13550-1-AP)

    IF Staining of HeLa using 13550-1-AP (same clone as 13550-1-PBS)

    Immunofluorescent analysis of Hela cells, using SFI1 antibody 13550-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13550-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SFI1 Polyclonal antibody, PBS Only

SFI1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13550-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

hSFI1, KIAA0542, Protein SFI1 homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13550-1-PBS targets SFI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4268

Product name: Recombinant human SFI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 917-1211 aa of BC033613

Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC

相同性解析による交差性が予測される生物種
Full Name Sfi1 homolog, spindle assembly associated (yeast)
Calculated molecular weight 1211 aa, 134 kDa
Observed molecular weight 148 kDa
GenBank accession numberBC033613
Gene Symbol SFI1
Gene ID (NCBI) 9814
RRIDAB_10596938
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA8K8P3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. SFI1 forms an interdependent complex with centrin at the centriolar distal end. Human SFI1 is an evolutionarily conserved protein of 1,242 amino acids that contains five residues within full Cdk consensus motifs (S/T*-P-x-K/R), four at the C terminus known to be phosphorylated in vivo (T816, S855, S882, S892) and one immediately preceding the C terminus (PMID: 25340401, 31197030).

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