Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of A549 using 13550-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 13550-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13550-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 13550-1-AP
Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13550-1-AP
Immunofluorescent analysis of Hela cells, using SFI1 antibody 13550-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

SFI1 Polyclonal antibody

SFI1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13550-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

Protein SFI1 homolog, KIAA0542, hSFI1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HepG2 cells, mouse lung tissue
Positive IF/ICC detected in	HepG2 cells, Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

13550-1-AP targets SFI1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4268 Product name: Recombinant human SFI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 917-1211 aa of BC033613 Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC 相同性解析による交差性が予測される生物種
Full Name	Sfi1 homolog, spindle assembly associated (yeast)
Calculated molecular weight	1211 aa, 134 kDa
Observed molecular weight	148 kDa
GenBank accession number	BC033613
Gene Symbol	SFI1
Gene ID (NCBI)	9814
RRID	AB_10596938
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	A8K8P3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for SFI1 antibody 13550-1-AP	Download protocol
WB protocol for SFI1 antibody 13550-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	CoIP	EBioMedicine PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response Authors - Wei Yue View Article
human	WB	J Cell Biol SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL. Authors - Andrew Kodani View Article
human	IF	J Cell Biol CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity Authors - Melis D Arslanhan View Article
human	WB	Sci Signal Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. Authors - Isha Nasa View Article
mouse	WB	Sci Rep Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation. Authors - Akiko Matsuura View Article
human	WB	J Cell Sci Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair. Authors - Ebtissal M Khouj View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 13550-1-AP

WB analysis of mouse lung using 13550-1-AP

WB analysis of HepG2 using 13550-1-AP

IF Staining of HepG2 using 13550-1-AP

IF Staining of HeLa using 13550-1-AP

SFI1 Polyclonal antibody

SFI1 Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response

SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL.

CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins.

Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation.

Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.