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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SETD7 Polyclonal antibody (24840-1-AP)

    WB analysis using 24840-1-AP (same clone as 24840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using SETD7 Polyclonal antibody (24840-1-AP)

    WB analysis of C6 using 24840-1-AP (same clone as 24840-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SETD7 Polyclonal antibody (24840-1-AP)

    WB analysis of mouse brain using 24840-1-AP (same clone as 24840-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using SETD7 Polyclonal antibody (24840-1-AP)

    WB analysis of mouse kidney using 24840-1-AP (same clone as 24840-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SETD7 Polyclonal antibody (24840-1-AP)

    IF Staining of HeLa using 24840-1-AP (same clone as 24840-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SETD7 Polyclonal antibody, PBS Only

SETD7 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24840-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24840-1-PBS targets SETD7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18219

Product name: Recombinant human SETD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-366 aa of BC121055

Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

相同性解析による交差性が予測される生物種
Full Name SET domain containing (lysine methyltransferase) 7
Calculated molecular weight 366 aa, 41 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC121055
Gene Symbol SETD7
Gene ID (NCBI) 80854
RRIDAB_2737406
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTS6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

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