Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, C6 cells, mouse brain tissue, mouse kidney tissue, L02 cells, rat brain tissue, NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 11 publications below |
| IHC | See 3 publications below |
| CoIP | See 3 publications below |
Product Information
24840-1-AP targets SETD7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18219 Product name: Recombinant human SETD7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC121055 Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK 相同性解析による交差性が予測される生物種 |
| Full Name | SET domain containing (lysine methyltransferase) 7 |
| Calculated molecular weight | 366 aa, 41 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC121055 |
| Gene Symbol | SETD7 |
| Gene ID (NCBI) | 80854 |
| RRID | AB_2737406 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WTS6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SETD7 antibody 24840-1-AP | Download protocol |
| WB protocol for SETD7 antibody 24840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Dynamic hydrogels orchestrate the differentiation fate of mesenchymal stem cells through epigenetic regulation of SETD7 to accelerate bone defect repair. | ||
Cell Death Dis ACAT2 suppresses the ubiquitination of YAP1 to enhance the proliferation and metastasis ability of gastric cancer via the upregulation of SETD7
| ||
Mol Ther Integrated stress response promotes acute liver failure by activating SETD7 and enhancing NLRP3 methylation. | ||
EBioMedicine Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients. | ||
Acta Pharmacol Sin Inhibition of Setd7 protects against cardiomyocyte hypertrophy via inhibiting lipid oxidation.
| ||
Biol Direct SETD7-mediated epigenetic regulation of LncRNA XIST promotes trophoblast pyroptosis: a study in preeclampsia mouse and cell models. |