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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SETD3 Polyclonal antibody (28692-1-AP)

    WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SETD3 Polyclonal antibody (28692-1-AP)

    IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SETD3 Polyclonal antibody (28692-1-AP)

    IHC staining of mouse liver using 28692-1-AP (same clone as 28692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SETD3 Polyclonal antibody (28692-1-AP)

    IF Staining of MCF-7 using 28692-1-AP (same clone as 28692-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SETD3 Polyclonal antibody (28692-1-AP)

    IF Staining of SH-SY5Y using 28692-1-AP (same clone as 28692-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

SETD3 Polyclonal antibody, PBS Only

SETD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28692-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C14orf154, SET domain containing 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28692-1-PBS targets SETD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30017

Product name: Recombinant human SETD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of NM_032233

Sequence: MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELF

相同性解析による交差性が予測される生物種
Full Name SET domain containing 3
Calculated molecular weight 67 kDa
Observed molecular weight67 kDa
GenBank accession numberNM_032233
Gene Symbol SETD3
Gene ID (NCBI) 84193
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TU7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).

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