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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SETD3 Polyclonal antibody

SETD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28692-1-AP

Synonyms

C14orf154, DKFZp761E1415, FLJ23027, SET domain containing 3, SETD3

Validation Data Gallery

Applications:
  • WB analysis using 28692-1-AP

    WB analysis using 28692-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 28692-1-AP

    IHC staining of mouse small intestine using 28692-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SETD3 antibody included
  • IHC staining of mouse liver using 28692-1-AP

    IHC staining of mouse liver using 28692-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SETD3 antibody included

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inmouse small intestine tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SETD3 Ready-To-Use IHC Kit. SETD3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28692-1-AP targets SETD3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SETD3 fusion protein Ag30017 相同性解析による交差性が予測される生物種
Full Name SET domain containing 3
Calculated molecular weight 67 kDa
Observed molecular weight67 kDa
GenBank accession numberNM_032233
Gene symbol SETD3
Gene ID (NCBI) 84193
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).

Protocols

Product Specific Protocols
WB protocol for SETD3 antibody 28692-1-APDownload protocol
IHC protocol for SETD3 antibody 28692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols