Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67936 targets SETD1A in applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SETD1A fusion protein Ag30424 相同性解析による交差性が予測される生物種 |
| Full Name | SET domain containing 1A |
| Calculated molecular weight | 186 kDa |
| Observed molecular weight | 270 kDa |
| GenBank accession number | NM_014712 |
| Gene Symbol | SETD1A |
| Gene ID (NCBI) | 9739 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15047 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).