Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of human plasma using 12010-1-AP (same clone as 12010-1-PBS)
human plasma were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
WB analysis of A375 using 12010-1-AP (same clone as 12010-1-PBS)
A375 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 12010-1-AP (same clone as 12010-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 12010-1-AP (same clone as 12010-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12010-1-AP (same clone as 12010-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12010-1-AP (SERPINA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12010-1-PBS in a different storage buffer formulation.

SERPINA6/CBG Polyclonal antibody, PBS Only

SERPINA6/CBG Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12010-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12010-1-PBS targets SERPINA6/CBG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2623 Product name: Recombinant human Corticosteroid binding globulin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-406 aa of BC058021 Sequence: QAMDPNAAYVNMSNHHRGLASANVDFAFSLYKHLVALSPKKNIFISPVSISMALAMLSLGTCGHTRAQLLQGLGFNLTERSETEIHQGFQHLHQLFAKSDTSLEMTMGNALFLDGSLELLESFSADIKHYYESEVLAMNFQDWATASRQINSYVKNKTQGKIVDLFSGLDSPAILVLVNYIFFKGTWTQPFDLASTREENFYVDETTVVKVPMMLQSSTISYLHDAELPCQLVQMNYVGNGTVFFILPDKGKMNTVIAALSRDTINRWSAGLTSSQVDLYIPKVTISGVYDLGDVLEEMGIADLFTNQANFSRITQDAQLKSSKVVHKAVLQLNEEGVDTAGSTGVTLNLTSKPIILRFNQPFIIMIFDHFTWSSLFLARVMNPV 相同性解析による交差性が予測される生物種
Full Name	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
Calculated molecular weight	405 aa, 45 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC058021
Gene Symbol	SERPINA6
Gene ID (NCBI)	866
RRID	AB_874108
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08185
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human plasma using 12010-1-AP (same clone as 12010-1-PBS)

WB analysis of A375 using 12010-1-AP (same clone as 12010-1-PBS)

WB analysis of HEK-293 using 12010-1-AP (same clone as 12010-1-PBS)

WB analysis of L02 using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human kidney using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human kidney using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human liver using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human liver using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human hepatocirrhosis using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human hepatocirrhosis using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)

IHC staining of human liver cancer using 12010-1-AP (same clone as 12010-1-PBS)

IF Staining of HepG2 using 12010-1-AP (same clone as 12010-1-PBS)

SERPINA6/CBG Polyclonal antibody, PBS Only

SERPINA6/CBG Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

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