Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue, A375 cells, HEK-293 cells, L02 cells |
| Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue, human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12010-1-AP targets SERPINA6/CBG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SERPINA6/CBG fusion protein Ag2623 相同性解析による交差性が予測される生物種 |
| Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6 |
| Calculated molecular weight | 405 aa, 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC058021 |
| Gene Symbol | SERPINA6 |
| Gene ID (NCBI) | 866 |
| RRID | AB_874108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08185 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINA6/CBG antibody 12010-1-AP | Download protocol |
| IHC protocol for SERPINA6/CBG antibody 12010-1-AP | Download protocol |
| IF protocol for SERPINA6/CBG antibody 12010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Food Funct Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism | ||
PLoS One Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. |