Various lysates were subjected to SDS PAGE followed by western blot with 30146-1-AP (SEPT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

IP result of anti-SEPT3 (IP:30146-1-AP, 4ug; Detection:30146-1-AP 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.

1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEPT3 (30146-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 30146-1-PBS in a different storage buffer formulation.