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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT3 Polyclonal antibody (30146-1-AP)

    WB analysis using 30146-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30146-1-AP (SEPT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEPT3 Polyclonal antibody (30146-1-AP)

    IP experiment of mouse brain using 30146-1-AP

    IP result of anti-SEPT3 (IP:30146-1-AP, 4ug; Detection:30146-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEPT3 Polyclonal antibody (30146-1-AP)

    IHC staining of mouse brain using 30146-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using SEPT3 Polyclonal antibody (30146-1-AP)

    IHC staining of rat brain using 30146-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using SEPT3 Polyclonal antibody (30146-1-AP)

    FC experiment of HEK-293 using 30146-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEPT3 (30146-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SEPT3 Polyclonal antibody

SEPT3 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 30146-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

bK250D10.3, Neuronal specific septin 3, Neuronal-specific septin-3, SEP3, septin 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30146-1-AP targets SEPT3 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32890

Product name: Recombinant human SEPT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 264-345 aa of BC111779

Sequence: VLGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGGLPPGEGLLGTVLPPVPATPCPTAE

相同性解析による交差性が予測される生物種
Full Name septin 3
Calculated molecular weight 358 aa, 41 kDa
Observed molecular weight40 kDa
GenBank accession numberBC111779
Gene Symbol SEPT3
Gene ID (NCBI) 55964
RRIDAB_2935520
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UH03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEPT3 is a developmentally regulated phosphoprotein. SEPT3 is a member of the septin GTPase family, which can form polymers and contribute to the cytoskeleton. SEPT3 is involved in neuronal autophagy. In the process of autophagy regulation, the level of SEPT3 will change according to the autophagy protein. SEPT3 is specifically expressed in the brain (PMID: 15485489; 35932293).

Protocols

Product Specific Protocols
FC protocol for SEPT3 antibody 30146-1-APDownload protocol
IHC protocol for SEPT3 antibody 30146-1-APDownload protocol
IP protocol for SEPT3 antibody 30146-1-APDownload protocol
WB protocol for SEPT3 antibody 30146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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