Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60075 targets Septin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1946 Product name: Recombinant human SEPT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC014455 Sequence: MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVISGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV 相同性解析による交差性が予測される生物種 |
| Full Name | septin 2 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 42-45 kDa |
| GenBank accession number | BC014455 |
| Gene Symbol | Septin 2 |
| Gene ID (NCBI) | 4735 |
| RRID | AB_2883416 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15019 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Septin 2 antibody CL594-60075 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
