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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SEPN1 Polyclonal antibody (55333-1-AP)

    WB analysis of A549 using 55333-1-AP (same clone as 55333-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SEPN1 Polyclonal antibody (55333-1-AP)

    WB analysis using 55333-1-AP (same clone as 55333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of rat skeletal muscle using 55333-1-AP (same clone as 55333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse uterus tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse uterus using 55333-1-AP (same clone as 55333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse lung using 55333-1-AP (same clone as 55333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse skeletal muscle using 55333-1-AP (same clone as 55333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SEPN1 Polyclonal antibody (55333-1-AP)

    IHC staining of mouse skeletal muscle using 55333-1-AP (same clone as 55333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55333-1-PBS in a different storage buffer formulation.

SEPN1 Polyclonal antibody, PBS Only

SEPN1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55333-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

MDRS1, RSMD1, RSS, SELENON, Selenoprotein N

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55333-1-PBS targets SEPN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name selenoprotein N, 1
Calculated molecular weight 66 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_020451
Gene Symbol SEPN1
Gene ID (NCBI) 57190
RRIDAB_2881308
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZV5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SEPN1 (Selenoprotein N, 1) is a selenoprotein that plays a crucial role in maintaining cellular redox homeostasis and calcium homeostasis. It is encoded by the SEPN1 gene and is widely expressed in various tissues, particularly in skeletal muscle. (PMID: 32661288)

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