Validation Data Gallery

Filter: AllWBIHC

WB analysis of A549 using 55333-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of rat skeletal muscle using 55333-1-AP
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse uterus using 55333-1-AP
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 55333-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 55333-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 55333-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEPN1 Polyclonal antibody

SEPN1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55333-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MDRS1, RSMD1, RSS, SELENON, Selenoprotein N, selenoprotein N, 1, SELN, SEPN1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells
Positive IHC detected in	mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse uterus tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below

Product Information

55333-1-AP targets SEPN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	selenoprotein N, 1
Calculated molecular weight	66 kDa
Observed molecular weight	70 kDa
GenBank accession number	NM_020451
Gene Symbol	SEPN1
Gene ID (NCBI)	57190
RRID	AB_2881308
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZV5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SEPN1 antibody 55333-1-AP	Download protocol
IHC protocol for SEPN1 antibody 55333-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne) Changes and related factors of blood CCN1 levels in diabetic patients Authors - Zhao-Yu Xiang View Article
human	WB	Antioxidants (Basel) Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco. Authors - Dian-Guang Zhang View Article
mouse	WB	Sci Rep Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins. Authors - Jiayong Tang View Article
mouse	WB	Mar Drugs Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice. Authors - Chih-Hung Guo View Article
mouse	WB	Front Nutr Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation. Authors - Arlene C P Kiyohara View Article
	WB	Br J Nutr Dietary selenium (Se) sources differentially regulate Se concentration, mRNA and protein expression of representative selenoproteins in various tissues of yellow catfish Pelteobagrus fulvidraco. Authors - Xiao-Jian Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 55333-1-AP

IHC staining of rat skeletal muscle using 55333-1-AP

IHC staining of mouse uterus using 55333-1-AP

IHC staining of mouse lung using 55333-1-AP

IHC staining of mouse skeletal muscle using 55333-1-AP

IHC staining of mouse skeletal muscle using 55333-1-AP

SEPN1 Polyclonal antibody

SEPN1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Changes and related factors of blood CCN1 levels in diabetic patients

Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.

Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins.

Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.

Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation.

Dietary selenium (Se) sources differentially regulate Se concentration, mRNA and protein expression of representative selenoproteins in various tissues of yellow catfish Pelteobagrus fulvidraco.