Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, SK-N-SH cells |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84080-3-RR targets SEMA3B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag25163 Product name: Recombinant human SEMA3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-629 aa of BC009113 Sequence: TLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERTARGLLLRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGGGGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGP 相同性解析による交差性が予測される生物種 |
| Full Name | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B |
| Observed molecular weight | 83 kDa |
| GenBank accession number | BC009113 |
| Gene Symbol | SEMA3B |
| Gene ID (NCBI) | 7869 |
| RRID | AB_3671648 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q13214 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SEMA3B antibody 84080-3-RR | Download protocol |
| WB protocol for SEMA3B antibody 84080-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |