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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA3B Recombinant antibody (84080-3-RR)

    WB analysis using 84080-3-RR (same clone as 84080-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84080-3-RR (SEMA3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84080-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SEMA3B Recombinant antibody (84080-3-RR)

    IF Staining of U-251 using 84080-3-RR (same clone as 84080-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SEMA3B antibody (84080-3-RR, Clone: 241171A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84080-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84080-3-RR

    Affinity and Kinetic Characterization of 84080-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84080-3-RR against Human SEMA3B were performed. The affinity constant is 8.53 nM.

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SEMA3B Recombinant antibody, PBS Only

SEMA3B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84080-3-PBS
Clone No.241171A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

241171A7, LUCA 1, SemA, Sema A(V), Sema V

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84080-3-PBS targets SEMA3B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEMA3B fusion protein Ag25163 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Observed molecular weight83 kDa
GenBank accession numberBC009113
Gene Symbol SEMA3B
Gene ID (NCBI) 7869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13214
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560).