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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEL1L3 Polyclonal antibody (31489-1-AP)

    WB analysis using 31489-1-AP (same clone as 31489-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31489-1-AP (SEL1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Daudi cells using SEL1L3 Polyclonal antibody (31489-1-AP)

    IP experiment of Daudi using 31489-1-AP (same clone as 31489-1-PBS)

    IP result of anti-SEL1L3 (IP:31489-1-AP, 4ug; Detection:31489-1-AP 1:1000) with Daudi cells lysate 1600 ug. This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of U-251 cells using SEL1L3 Polyclonal antibody (31489-1-AP)

    IP experiment of U-251 using 31489-1-AP (same clone as 31489-1-PBS)

    IP result of anti-SEL1L3 (IP:31489-1-AP, 4ug; Detection:31489-1-AP 1:1000) with U-251 cells lysate 1320 ug. This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SEL1L3 Polyclonal antibody (31489-1-AP)

    IHC staining of human colon cancer using 31489-1-AP (same clone as 31489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31489-1-AP (SEL1L3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31489-1-PBS in a different storage buffer formulation.

SEL1L3 Polyclonal antibody, PBS Only

SEL1L3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 31489-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

KIAA0746, Protein sel 1 homolog 3, Sel 1L3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31489-1-PBS targets SEL1L3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34856

Product name: Recombinant human SEL1L3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-236 aa of BC060867

Sequence: KYSGRDWNVKWEENLLHAVAKNYTLLQTIPPFERPFKDHQVCLEWNMGYIWNLRANRIPQCPLENDVVALLGFPYASSGENTGIVKKFPRFRNRELEATRRQRMDYPVFTVSLWLYLLHYCKANLCGILYFVDSNEMYGTPSVFLTEEGYLHIQMHLVKGEDLAVKTKFIIPLKEWFRLDISFNGGQIVVTTSIGQDLKSYHNQTISFRE

相同性解析による交差性が予測される生物種
Full Name KIAA0746 protein
Observed molecular weight 129 kDa
GenBank accession numberBC060867
Gene Symbol KIAA0746
Gene ID (NCBI) 23231
RRIDAB_3742437
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ68CR1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SEL1L3 is also named suppressor of lin-12-like protein 3. SEL1L3, a protein with multiple glycosylation sites, was identified as the BCR target in 3/20 PVRL cases. SEL1L3 induces proliferation and BCR pathway activation in aggressive lymphoma cell lines. Moreover, SEL1L3 conjugated to a toxin killed exclusively lymphoma cells with respective BCR-reactivity (PMID: 38671086).

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